5LYP

Crystal structure of the Tpr Domain of Sgt2.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2016-03-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.920
Spacegroup name	P 21 21 21
Unit cell lengths	36.861, 50.760, 67.116
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.558 - 1.550
R-factor	0.1948
Rwork	0.194
R-free	0.21850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vyi
RMSD bond length	0.005
RMSD bond angle	0.619
Data reduction software	xia2
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.860	36.860	1.590
High resolution limit [Å]	1.550	6.930	1.550
Rmerge	0.136	0.094	0.562
Number of reflections	18957
<I/σ(I)>	13.9
Completeness [%]	100.0	99.5	100
Redundancy	12.2	10.7	12.4
CC(1/2)	0.996	0.990	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	6	293.15	0.1 M SPG, pH 6.0, 25 % w/v PEG 1500