5LYB

Crystal structure of the S.cerevisiae 80S ribosome in complex with the A-site bound aminoacyl-tRNA analog ACCPmn

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97900
Spacegroup name	P 1 21 1
Unit cell lengths	436.180, 288.240, 303.580
Unit cell angles	90.00, 98.87, 90.00

Refinement procedure

Resolution	172.587 - 3.250
R-factor	0.2086
Rwork	0.208
R-free	0.24880
RMSD bond length	0.009
RMSD bond angle	1.262
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.10.1_2155)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	200.000		3.300
High resolution limit [Å]	3.250	150.000	3.250
Rmerge	0.422	0.015	5.801
Number of reflections	1159737
<I/σ(I)>	7.87	26.61	0.99
Completeness [%]	100.0	23.1	100
Redundancy	5
CC(1/2)	0.998	1.000	0.387

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	277	Tris-Acetate pH 7.0, KSCN, MgOAc2, glycerol, spermidine, PEG20K