5LXX

High-resolution structure of human collapsin response mediator protein 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03
Spacegroup name	C 1 2 1
Unit cell lengths	141.760, 97.040, 92.380
Unit cell angles	90.00, 122.54, 90.00

Refinement procedure

Resolution	75.332 - 1.250
R-factor	0.1038
Rwork	0.103
R-free	0.13260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gse
RMSD bond length	0.013
RMSD bond angle	1.269
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2247: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.280
High resolution limit [Å]	1.250	1.250
Rmerge	0.039	0.770
Number of reflections	269401
<I/σ(I)>	15.9	1
Completeness [%]	92.7	57.9
Redundancy	3.6	2.3
CC(1/2)	1.000	0.399

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	bis-tris, PEG3350, ammonium sulphate, glycerol