5LXR
Structure of the minimal RBM7 - ZCCHC8 Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.6 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 79.519, 79.519, 87.609 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 87.610 - 2.000 |
Rwork | 0.202 |
R-free | 0.22860 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol (1.10.1) |
Refinement software | PHENIX (1.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.610 | 2.080 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.187 | |
Number of reflections | 11470 | |
<I/σ(I)> | 26.6 | |
Completeness [%] | 98.8 | 92.7 |
Redundancy | 134.8 | |
CC(1/2) | 1.000 | 0.341 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | 0.1 M Bis-Tris-Propane pH 6.5 0.2 M NaBr 18% (v/v) PEG3350 7% (w/v) glycerol |