5LXQ
Structure of PRL-1 in complex with the Bateman domain of CNNM2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.2837 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.930, 125.560, 61.040 |
| Unit cell angles | 90.00, 111.43, 90.00 |
Refinement procedure
| Resolution | 47.882 - 3.335 |
| R-factor | 0.2056 |
| Rwork | 0.203 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4IY0 and 1ZCK |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.676 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.640 | 3.342 |
| High resolution limit [Å] | 3.100 | 3.331 |
| Rmerge | 0.084 | 0.999 |
| Number of reflections | 10602 | |
| <I/σ(I)> | 16 | |
| Completeness [%] | 99.2 | |
| Redundancy | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 2 M Sodium formate, 0.1 M Sodium acetate pH 4.6 |
