5LXH
GABARAP-L1 ATG4B LIR Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.968 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.520, 79.885, 97.643 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.822 - 1.580 |
| R-factor | 0.2069 |
| Rwork | 0.205 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r2q |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.428 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.000 | 1.600 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.870 | |
| Number of reflections | 55946 | |
| <I/σ(I)> | 10.7 | 1.3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 5.1 | 4.9 |
| CC(1/2) | 0.990 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.17 M AMSO4, 40 % PEG 4000, 15 % glycerol |
