5LXE
F420-dependent glucose-6-phosphate dehydrogenase from Rhodococcus jostii RHA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-31 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.71, 2.25 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.540, 88.120, 88.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.540 - 1.470 |
R-factor | 0.15778 |
Rwork | 0.156 |
R-free | 0.18530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b4y |
RMSD bond length | 0.021 |
RMSD bond angle | 2.020 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.780 | 1.500 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmerge | 0.050 | 0.550 |
Number of reflections | 106774 | |
<I/σ(I)> | 10.9 | |
Completeness [%] | 98.7 | |
Redundancy | 3.9 | |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | ammonium sulfate, sodium acetate pH 4.6, PEG 4000 |