5LXC
Crystal structure of DYRK2 in complex with EHT 5372 (Compound 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-19 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9200 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.220, 60.980, 148.790 |
| Unit cell angles | 90.00, 105.04, 90.00 |
Refinement procedure
| Resolution | 49.430 - 2.150 |
| R-factor | 0.20808 |
| Rwork | 0.206 |
| R-free | 0.25401 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.443 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.430 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.107 | 0.578 |
| Number of reflections | 60094 | |
| <I/σ(I)> | 8.2 | 2.1 |
| Completeness [%] | 97.6 | 98.7 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277.15 | 25% PEG 3350, 0.2 M NaCl and 0.1 M bis-tris, pH 5.5 |
