5LWV
Human OGT in complex with UDP and fused substrate peptide (HCF1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 168.024, 168.024, 162.265 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.500 - 1.900 |
| R-factor | 0.17094 |
| Rwork | 0.169 |
| R-free | 0.19933 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pe4 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.999 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.530 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.147 | 1.950 |
| Number of reflections | 106157 | |
| <I/σ(I)> | 14.4 | 1.7 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 20 | 20.2 |
| CC(1/2) | 0.998 | 0.684 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.3M Ammonium tartrate diabasic; 0.1M Tris pH8.5 |
