5LU0
Crystal structure of H. pylori referent strain in complex with PO4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97957 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.520, 86.310, 268.449 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 134.220 - 1.730 |
| R-factor | 0.1859 |
| Rwork | 0.184 |
| R-free | 0.22770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PNP from E. Coli |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.726 |
| Data reduction software | iMOSFLM |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 134.220 | 1.790 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Number of reflections | 133010 | |
| <I/σ(I)> | 28.88 | 3.86 |
| Completeness [%] | 87.0 | 92 |
| Redundancy | 11 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1 M Tris, 10 % PEG 8000 |
