5LTL
Structure of human chemokine CCL16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.350, 24.590, 37.970 |
| Unit cell angles | 90.00, 93.12, 90.00 |
Refinement procedure
| Resolution | 37.910 - 1.450 |
| R-factor | 0.1719 |
| Rwork | 0.171 |
| R-free | 0.19760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q8t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.049 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.910 | 1.490 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.057 | 0.529 |
| Number of reflections | 22666 | |
| <I/σ(I)> | 12.6 | 2.3 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 5 | 5.1 |
| CC(1/2) | 0.998 | 0.883 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.05 M sodium phosphate, 0.5 M sodium chloride, 10 % (v/v) glycerol, 1.6 M sodium citrate |
