5LT6
Structure of the Epigenetic Oncogene MMSET and inhibition by N-Alkyl Sinefungin Derivatives
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.360, 72.700, 61.860 |
| Unit cell angles | 90.00, 89.77, 90.00 |
Refinement procedure
| Resolution | 61.860 - 2.050 |
| R-factor | 0.182 |
| Rwork | 0.179 |
| R-free | 0.23100 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 61.860 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 98247 |
| <I/σ(I)> | 6.1 |
| Completeness [%] | 98.1 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1MHepes,pH7.3, 0.1MKSCN, 25-30%MPEG2000. |
