5LT3

nucleotide-free kinesin-1 motor domain T87A mutant, P1 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	56.672, 101.299, 101.527
Unit cell angles	119.57, 91.91, 91.75

Refinement procedure

Resolution	33.176 - 2.590
R-factor	0.2061
Rwork	0.203
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lnu
RMSD bond length	0.009
RMSD bond angle	1.369
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900
High resolution limit [Å]	2.590	2.590
Number of reflections	59275
<I/σ(I)>	3.8
Completeness [%]	97.3
Redundancy	2.9
CC(1/2)	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	50 mM Mes pH 6.5 0.2M ammonium sulfate 30% mPEG 5000