5LT0

nucleotide-free kinesin-1 motor domain, P212121 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	49.029, 68.058, 112.378
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.938 - 2.000
R-factor	0.1771
Rwork	0.174
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bg2
RMSD bond length	0.007
RMSD bond angle	0.857
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11rc1_2513: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.940
High resolution limit [Å]	2.000
Rmeas	0.170	1.380
Number of reflections	26061
<I/σ(I)>	9.23
Completeness [%]	99.8
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M Na-acetate pH 4.5-5.5 20-25% PEG 4000 0.2 M ammonium sulfate 0.1 mM SrCl2