5LSW

A CAF40-binding motif facilitates recruitment of the CCR4-NOT complex to mRNAs targeted by Drosophila Roquin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00001
Spacegroup name	P 1 21 1
Unit cell lengths	56.930, 103.690, 60.830
Unit cell angles	90.00, 113.00, 90.00

Refinement procedure

Resolution	46.769 - 2.150
R-factor	0.1861
Rwork	0.184
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fv2 CHAIN A
RMSD bond length	0.004
RMSD bond angle	0.597
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.800	2.200
High resolution limit [Å]	2.150	2.150
Number of reflections	34975
<I/σ(I)>	13.6	2.2
Completeness [%]	98.7	98.8
Redundancy	2.9	2.7
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	0.1 M Sodium Acetate pH 5.0 17.5 % (w/v) PEG 4000 0.1 M Ammonium sulfate