5LST

Crystal structure of the human RecQL4 helicase.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9763
Spacegroup name	P 31 2 1
Unit cell lengths	131.001, 131.001, 96.344
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.340 - 2.750
R-factor	0.1853
Rwork	0.183
R-free	0.23140
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.681
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.340	2.900
High resolution limit [Å]	2.750	2.750
Rmerge	0.070	1.894
Number of reflections	25149
<I/σ(I)>	21.7	1.4
Completeness [%]	99.8	100
Redundancy	14.3	14.3
CC(1/2)	0.999	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293.15	Imidazole, Sodium formate