5LSR

Carboxysome shell protein CcmP from Synechococcus elongatus PCC 7942

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97625
Spacegroup name	I 21 3
Unit cell lengths	178.740, 178.740, 178.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.680 - 1.650
R-factor	0.1818
Rwork	0.181
R-free	0.19110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ht5
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	XDS (November 3, 2014)
Data scaling software	Aimless (0.1.29)
Phasing software	PHASER (2.5.2)
Refinement software	BUSTER-TNT (1.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.690	44.690	1.680
High resolution limit [Å]	1.650	9.040	1.650
Rmerge	0.093	0.071	1.404
Number of reflections	113271
<I/σ(I)>	18.9	48.3	2.3
Completeness [%]	100.0	99.2	100
Redundancy	20.1	19.7	17.6
CC(1/2)	0.999	0.998	0.764

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20 mM BIS-tris propane, 50 mM NaCl, 0.1 mM RuBP, 0.1 mM TrisHCl pH 7, 0.2 mM potassium thiocyanate, 6% poly-alpha-glutamic acid,