5LSK

CRYSTAL STRUCTURE OF THE HUMAN KINETOCHORE MIS12-CENP-C COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97863
Spacegroup name	C 1 2 1
Unit cell lengths	146.010, 112.690, 90.810
Unit cell angles	90.00, 114.18, 90.00

Refinement procedure

Resolution	19.742 - 3.502
R-factor	0.2471
Rwork	0.244
R-free	0.29770
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.630
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.742		3.590
High resolution limit [Å]	3.500	15.650	3.500
Rmerge	0.181	0.050	1.956
Number of reflections	16932
<I/σ(I)>	7.16	28.52	1.05
Completeness [%]	99.0	49	99.1
Redundancy	6.76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	6-12% PEG6000