5LSF

Sacbrood honeybee virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97857
Spacegroup name	C 1 2 1
Unit cell lengths	487.850, 360.970, 338.160
Unit cell angles	90.00, 133.07, 90.00

Refinement procedure

Resolution	50.000 - 2.100
Rwork	0.194
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b35
RMSD bond length	0.006
RMSD bond angle	1.440
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.135	0.639
Number of reflections	1572113
<I/σ(I)>	6.6	1
Completeness [%]	64.0	21.5
Redundancy	3.1	1.4
CC(1/2)	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277.15	PEG4000, isopropanol, magnesium chloride, MES