5LSE
PHOTOSYNTHETIC REACTION CENTER MUTANT WITH Glu L212 replaced with Ala (CHAIN L, EL212W), Asp L213 replaced with ALA (Chain L, DL213A) AND LEU M215 REPLACED WITH ALA (CHAIN M, LM215A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-11 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 138.993, 138.993, 184.711 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.900 - 2.500 |
| R-factor | 0.16934 |
| Rwork | 0.168 |
| R-free | 0.20118 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WILD TYPE RHODOBACTER SPHAEROIDES COORDINATES (Unpublished data) |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.836 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.900 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.530 |
| Number of reflections | 70501 | |
| <I/σ(I)> | 16 | 2.7 |
| Completeness [%] | 98.4 | 97.9 |
| Redundancy | 5.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | Well containing 9 mg mL-1 RC, 0.09 % v/v LDAO, 3.5 % w/v 1,2,3-heptanetriol, and 0.75 M potassium phosphate (pH 7.5) equilibrated against 1.5 M potassium phosphate |
