5LS7
Complex of wild type E. coli alpha aspartate decarboxylase with its processing factor PanZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | I 4 |
| Unit cell lengths | 85.863, 85.863, 80.055 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.280 - 1.160 |
| R-factor | 0.11458 |
| Rwork | 0.113 |
| R-free | 0.13688 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cry |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.851 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.280 | 1.180 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmerge | 0.077 | 0.395 |
| Number of reflections | 99822 | |
| <I/σ(I)> | 9 | 2.1 |
| Completeness [%] | 99.6 | 93.7 |
| Redundancy | 5.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 200 mM KSCN, 100 mM Bis-Tris propane pH 6.5, 20% v/v PEG 3350 |
