5LS1
CRYSTAL STRUCTURE OF HSP90 IN COMPLEX WITH SAR166475
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 66.424, 98.058, 90.014 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.530 - 1.850 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yk2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.200 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 25349 |
| <I/σ(I)> | 27.2 |
| Completeness [%] | 99.0 |
| Redundancy | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1.0-1.4M Sodium Citrate |
