5LRX
Structure of A20 OTU domain bound to ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96863 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.195, 71.950, 203.926 |
| Unit cell angles | 90.00, 94.64, 90.00 |
Refinement procedure
| Resolution | 49.325 - 2.850 |
| R-factor | 0.1977 |
| Rwork | 0.195 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.535 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.330 | 2.960 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.070 | 0.384 |
| Number of reflections | 43448 | |
| <I/σ(I)> | 9.6 | 2.1 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 287 | 0.1 M MES/imidazole (pH 6.5), 7% (w/v) PEG 8K, 20% ethylene glycol |
