5LRD
Crystal structure of Glycogen Phosphorylase b in complex with KS242
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2015-09-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0403 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.540, 128.540, 116.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.140 - 1.800 |
| R-factor | 0.1616 |
| Rwork | 0.160 |
| R-free | 0.18722 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.282 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.370 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 88657 | |
| <I/σ(I)> | 14.1 | 3.5 |
| Completeness [%] | 98.5 | 97.6 |
| Redundancy | 4.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.8 | 289 | 10mM BES buffer |
