5LRA
Plastidial phosphorylase PhoI from barley in complex with maltotetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87260 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 227.690, 63.150, 148.190 |
| Unit cell angles | 90.00, 114.72, 90.00 |
Refinement procedure
| Resolution | 49.390 - 3.000 |
| R-factor | 0.19626 |
| Rwork | 0.195 |
| R-free | 0.24286 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lrb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.158 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.109 | 1.128 |
| Number of reflections | 38803 | |
| <I/σ(I)> | 10.1 | 1.13 |
| Completeness [%] | 99.7 | |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 288 | 30% PEG4000, 0.1M Na Citrate |
