5LR4
RNA duplex has central consecutive GA pairs flanked by G-U basepairs with a methyl group on the adenine N6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9196 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 49.870, 49.870, 123.735 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.255 - 1.800 |
| R-factor | 0.2704 |
| Rwork | 0.269 |
| R-free | 0.28880 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 4.218 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.260 | 1.760 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.075 | 2.200 |
| Number of reflections | 17404 | |
| <I/σ(I)> | 9.5 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.7 | 12.2 |
| CC(1/2) | 1.000 | 0.898 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Sodium Chloride, 0.1M Bis-Tris pH 6.5, 1.5 M Ammonium Sulfate |
