5LQO

RNA duplex has central consecutive GA pairs flanked by G-C basepairs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9196
Spacegroup name	P 43 21 2
Unit cell lengths	33.469, 33.469, 113.766
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.108 - 1.870
R-factor	0.2461
Rwork	0.246
R-free	0.25560
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.458
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2481: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.470	1.920
High resolution limit [Å]	1.870	1.870
Rmerge	0.072	2.080
Number of reflections	5917
<I/σ(I)>	10.7	1.3
Completeness [%]	100.0	100
Redundancy	12.2	13
CC(1/2)	1.000	0.446

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.025 M Magnesium Sulfate, 0.05 M Tris Hydrochloride pH 8.5, 1.8 M Ammonium Sulfate