5LPN
Structure of human Rab10 in complex with the bMERB domain of Mical-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99997 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.390, 59.000, 198.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.004 - 2.800 |
| R-factor | 0.2462 |
| Rwork | 0.244 |
| R-free | 0.28660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lhw and 5szg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.118 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.004 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.118 | 0.723 |
| Number of reflections | 17508 | |
| <I/σ(I)> | 14.12 | 3.25 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 12.7 | 12.6 |
| CC(1/2) | 0.803 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 6%-10% PEG8000 0.1M Imidazole |
