5LPB

Crystal structure of the BRI1 kinase domain (865-1160) in complex with ADP from Arabidopsis thaliana

Replaces:  4OAC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97625
Spacegroup name	P 62
Unit cell lengths	116.141, 116.141, 49.743
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.580 - 1.980
R-factor	0.1838
Rwork	0.183
R-free	0.20320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uim
RMSD bond length	0.004
RMSD bond angle	0.792
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.580	2.100
High resolution limit [Å]	1.980	1.980
Number of reflections	26940
<I/σ(I)>	24.28	1.71
Completeness [%]	99.9	99.6
Redundancy	10.2	10.1
CC(1/2)	1.000	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	28% PEG 20K/MME 550 morpheus mix 0.12 M eth glycol mix 0.1 M Hepes pH 7 1mM ADP