5LP0
CRYSTAL STRUCTURE OF THE ZEBRA FISH ENTH DOMAIN FROM EPSIN1 IN 1.41 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97881 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.364, 51.724, 64.639 |
| Unit cell angles | 89.33, 76.37, 79.74 |
Refinement procedure
| Resolution | 40.490 - 1.410 |
| R-factor | 0.157 |
| Rwork | 0.154 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h0a |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.134 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.788 | 1.460 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.075 | 0.320 |
| Number of reflections | 95136 | |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 94.0 | 91.4 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 19% (W/V) PEG 3350, 0.1M POTASSIUM PHOSPHATE MONOBASIC MONOHYDRATE, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 292K |
