5LOP
Structure of the active form of /K. lactis/ Dcp1-Dcp2-Edc3 decapping complex bound to m7GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0725 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 219.810, 219.810, 74.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.200 - 3.500 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.26600 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.310 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 3.820 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.026 | 1.380 |
| Number of reflections | 13907 | |
| <I/σ(I)> | 19.4 | 0.9 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 21.2 | 21.2 |
| CC(1/2) | 0.998 | 0.362 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 25% PEG 400, 100 mM MgCl2 |
