5LOO
Structure of full length unliganded CodY from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98040 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 315.628, 113.676, 168.594 |
| Unit cell angles | 90.00, 113.23, 90.00 |
Refinement procedure
| Resolution | 19.880 - 4.500 |
| R-factor | 0.26008 |
| Rwork | 0.251 |
| R-free | 0.42963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5loj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.441 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.580 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.090 | 0.508 |
| Number of reflections | 29671 | |
| <I/σ(I)> | 6.77 | 1.01 |
| Completeness [%] | 71.4 | 71.4 |
| Redundancy | 3.9 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1M sodium citrate pH6.0, 5% glycerol |
