5LOI

Crystal structure of Myceliophthora thermophila Rad26 (residues 373-841)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	I 4 2 2
Unit cell lengths	210.783, 210.783, 104.766
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	105.391 - 3.153
R-factor	0.2101
Rwork	0.207
R-free	0.23510
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.010
Data reduction software	XDS
Phasing software	SHELXCD
Refinement software	PHENIX ((dev_2614: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.400	3.270
High resolution limit [Å]	3.150	3.150
Rmerge	0.123	4.781
Rpim	0.025	0.937
Number of reflections	20640	1932
<I/σ(I)>	23	0.8
Completeness [%]	99.4	95.07
Redundancy	26.2	26.8
CC(1/2)	0.999	0.612

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M BIS-TRIS pH 6.5 10% (w/v) PEG 10K 0.2M Potassium sodium tartrate