5LOD
Crystal structure of HhaI DNA methyltransferase in APO form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.000, 70.000, 87.800 |
| Unit cell angles | 90.00, 101.30, 90.00 |
Refinement procedure
| Resolution | 41.728 - 1.900 |
| R-factor | 0.1804 |
| Rwork | 0.178 |
| R-free | 0.21940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hmy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.077 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.730 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.058 | 0.429 |
| Number of reflections | 49539 | |
| <I/σ(I)> | 12.42 | 2.77 |
| Completeness [%] | 99.6 | 99.74 |
| Redundancy | 3.68 | 3.57 |
| CC(1/2) | 0.980 | 0.907 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | AMMONIUM SULFATE 50 mM, CITRATE 100mM, 32% (w/v) PEG-4000 |
