5LOC

Crystal structure of the engineered D-Amino Acid Dehydrogenase (DAADH)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9282
Spacegroup name	P 31 2 1
Unit cell lengths	120.530, 120.530, 97.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	71.302 - 2.040
R-factor	0.209
Rwork	0.207
R-free	0.25450
RMSD bond length	0.010
RMSD bond angle	1.319
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	71.302
High resolution limit [Å]	2.040
Number of reflections	529247
<I/σ(I)>	17.5
Completeness [%]	99.6
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.12 M monosaccharides, 0.1 M Buffer System 3 pH 8.5, 30%, P500MME_P20K