5LNU

Crystal structure of Arabidopsis thaliana Pdx1-I320 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-01-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91730
Spacegroup name	H 3
Unit cell lengths	178.117, 178.117, 115.963
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.346 - 1.730
R-factor	0.1638
Rwork	0.162
R-free	0.19120
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	0.813
Data scaling software	Aimless (0.5.17)
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	52.200	52.200	1.760
High resolution limit [Å]	1.730	9.480	1.730
Rmerge	0.054	0.021	0.445
Rmeas	0.066	0.025	0.548
Rpim	0.037	0.013	0.316
Total number of observations	408071	2821	20789
Number of reflections	141425
<I/σ(I)>	9.3	29.9	1.9
Completeness [%]	98.1	99.4	99.7
Redundancy	2.9	3.2	2.9
CC(1/2)	0.997	0.999	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1 M Tris pH8.5, 0.13 M sodium acetate pH5.5, 12.25%(w/v) PEG4000