5LNP
Domain-swapped dimer of human Dishevelled2 DEP domain: monoclinic crystal form crystallised from monomeric fraction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.928190 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.583, 60.181, 66.292 |
| Unit cell angles | 90.00, 117.71, 90.00 |
Refinement procedure
| Resolution | 42.020 - 1.990 |
| R-factor | 0.1963 |
| Rwork | 0.194 |
| R-free | 0.24552 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | unpublished model |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.743 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.020 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.064 | 0.636 |
| Number of reflections | 29398 | |
| <I/σ(I)> | 17.3 | 2.5 |
| Completeness [%] | 99.0 | |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES, pH=6.5 0.1 M NaCl 0.1 M Li2SO4 23 % PEG400 |
