5LND

Crystal structure of self-complemented MyfA, the major subunit of Myf fimbriae from Yersinia enterocolitica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	41.970, 41.979, 151.185
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.449 - 1.460
R-factor	0.1853
Rwork	0.181
R-free	0.19360
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.996
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.979	41.979	1.510
High resolution limit [Å]	1.460	5.650	1.460
Rmerge		0.049	0.474
Rmeas	0.074	0.057	0.561
Rpim	0.038	0.029	0.292
Total number of observations	149827	2841	15115
Number of reflections	45504
<I/σ(I)>	10.1	18	2.2
Completeness [%]	96.7	92.1	98.7
Redundancy	3.3	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	35% dioxane v/v