5LNC
Structure of SPX domain of the yeast inorganic polyphophate polymerase Vtc4 crystallized by carrier-driven crystallization in fusion with the macro domain of human histone macroH2A1.1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97963 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 114.020, 130.050, 158.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.900 - 3.290 |
R-factor | 0.28 |
Rwork | 0.279 |
R-free | 0.30200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yd9 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.130 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.900 | 3.380 |
High resolution limit [Å] | 3.290 | 3.290 |
Number of reflections | 17957 | |
<I/σ(I)> | 15.37 | 0.6 |
Completeness [%] | 98.4 | 82.3 |
Redundancy | 7.1 | 5.3 |
CC(1/2) | 1.000 | 0.253 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 8.75% PEG 6000, 2.75M NaCl |