5LN1

STRUCTURE OF UBIQUITYLATED-RPN10 FROM YEAST;

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9393
Spacegroup name	C 1 2 1
Unit cell lengths	107.290, 49.700, 81.330
Unit cell angles	90.00, 130.55, 90.00

Refinement procedure

Resolution	53.640 - 3.140
R-factor	0.193
Rwork	0.190
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x5n 1ubq
RMSD bond length	0.010
RMSD bond angle	1.409
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.800	3.310
High resolution limit [Å]	3.140	3.140
Rmerge	0.084	0.225
Number of reflections	5824
<I/σ(I)>	10.2	5.1
Completeness [%]	99.8	99.9
Redundancy	3.2	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION	6.5	292	12% (W/V) PEG 20000, 0.1M MES PH 6.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 292K