5LMG

Structure of C-terminal domain from S. cerevisiae Pat1 decapping activator bound to Dcp2 HLM10 peptide (region 954-970)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.28361
Spacegroup name	I 2 2 2
Unit cell lengths	97.686, 123.029, 126.562
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.820 - 1.890
R-factor	0.206
Rwork	0.204
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ogp
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER-TNT (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.890	1.890
Number of reflections	59110
<I/σ(I)>	12.15	0.6
Completeness [%]	97.2	88.4
Redundancy	4.2
CC(1/2)		0.459

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297.15	0.2 M MgCl2 ; 20 % PEG 3350