5LM5

Structure of C-terminal domain from S. cerevisiae Pat1 decapping activator bound to Dcp2 HLM2 peptide (region 435-451)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97533
Spacegroup name	I 2 2 2
Unit cell lengths	98.280, 115.960, 122.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.140 - 2.600
R-factor	0.256
Rwork	0.256
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ogp
RMSD bond length	0.010
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER-TNT (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.750
High resolution limit [Å]	2.600	10.000	2.600
Rmerge	0.134	0.047	1.391
Number of reflections	21634
<I/σ(I)>	6.52	21.73	1.01
Completeness [%]	99.1	96.8	98.9
Redundancy	4.3
CC(1/2)	0.996	0.996	0.542

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297.15	0.2 M MgCl2 20%PEG 3350