5LKJ

Crystal structure of mouse CARM1 in complex with ligand SA684

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.954
Spacegroup name	P 21 21 2
Unit cell lengths	74.637, 98.161, 206.499
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.902 - 2.595
R-factor	0.1905
Rwork	0.189
R-free	0.21970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
RMSD bond length	0.004
RMSD bond angle	0.876
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (dev_1951)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.690
High resolution limit [Å]	2.595	2.600
Number of reflections	47431
<I/σ(I)>	24.9	4.6
Completeness [%]	99.9	100
Redundancy	7	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	297	100 mM BTP pH 7.5 30% PEG 1500