5LID
X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromopromazine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91983 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.720, 267.910, 111.330 |
| Unit cell angles | 90.00, 106.70, 90.00 |
Refinement procedure
| Resolution | 49.537 - 4.500 |
| R-factor | 0.2148 |
| Rwork | 0.212 |
| R-free | 0.25710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.219 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.540 |
| High resolution limit [Å] | 3.700 |
| Rmerge | 0.154 |
| Number of reflections | 69231 |
| <I/σ(I)> | 11.2 |
| Completeness [%] | 99.8 |
| Redundancy | 21.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM ammonium sulfate, 50 mM ADA pH 6.5, 9-12 % PEG4000 |
