5LHZ

PB3 Domain of Human PLK4 in Complex with Coiled-Coil Domain of STIL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 32 2 1
Unit cell lengths	68.360, 68.360, 137.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.223 - 2.510
R-factor	0.2513
Rwork	0.250
R-free	0.28210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Model based on 4YYP
RMSD bond length	0.002
RMSD bond angle	0.432
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.223
High resolution limit [Å]	2.510	2.510
Rmerge		1.312
Number of reflections	13265
<I/σ(I)>	12.1
Completeness [%]	99.7
Redundancy	6.2
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	292	150nl Protein solution (41.87 mg/ml protein in 20 mM Tris pH 7.5, 150 mM NaCl, 2 mM DTT) and 50 nl mother liquor (100mM MES pH 6.0, 191.7 mM Zn acetate, 10% Isopropanol)