5LHW

Central Coiled-Coil Domain of Human STIL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.800
Spacegroup name	P 42 21 2
Unit cell lengths	40.310, 40.310, 29.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.663 - 0.910
R-factor	0.1347
Rwork	0.135
R-free	0.13870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PolyAla helix based on 5AL6
RMSD bond length	0.020
RMSD bond angle	1.607
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.663
High resolution limit [Å]	0.910	0.910
Rmerge		0.668
Number of reflections	17643
<I/σ(I)>	24
Completeness [%]	99.1
Redundancy	11.2
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	292	150 nl protein solution (58.6 mg/ml protein in 20 mM Tris pH 7.5, 150 mM NaCl, 2 mM TCEP) and 150 nl of mother liquor (7 mM HEPES pH 8.2, 93 mM Tris pH 9.0, 55.36% v/v PEG 550 MME, 10% v/v glycerol)