5LGY

Lysine 120-acetylated P53 DNA binding domain in a complex with the BAX response element.

Replaces:  5BUB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.954
Spacegroup name	P 1 21 1
Unit cell lengths	67.703, 70.344, 104.995
Unit cell angles	90.00, 95.83, 90.00

Refinement procedure

Resolution	43.464 - 2.920
R-factor	0.2395
Rwork	0.238
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kmd
RMSD bond length	0.011
RMSD bond angle	1.303
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.650	3.090
High resolution limit [Å]	2.920	2.920
Rmerge	0.194
Number of reflections	21618
<I/σ(I)>	10.9
Completeness [%]	99.8	99.1
Redundancy	7.6	7.6
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.1	286	0.22 M Ammonium acetate, 10% w/v PEG 3350, tri-Sodium citrate pH 6.1