5LGV

GlgE isoform 1 from Streptomyces coelicolor E423A mutant soaked in maltooctaose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.920
Spacegroup name	P 41 21 2
Unit cell lengths	113.611, 113.611, 313.692
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.920 - 2.500
R-factor	0.1805
Rwork	0.179
R-free	0.20880
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4cn6
RMSD bond length	0.010
RMSD bond angle	1.431
Data reduction software	XDS
Data scaling software	Aimless (0.3.6)
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.920	49.920	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.094	0.031	0.904
Number of reflections	72101
<I/σ(I)>	17.9		2.3
Completeness [%]	100.0	99	100
Redundancy	13	13.5	8.2
CC(1/2)	0.999	0.999	0.628

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	NULL