5LGQ

Crystal structure of mouse CARM1 in complex with ligand P2C3s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.073
Spacegroup name	P 21 21 2
Unit cell lengths	74.706, 99.011, 207.306
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.152 - 2.110
R-factor	0.189
Rwork	0.187
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
RMSD bond length	0.002
RMSD bond angle	0.530
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2386: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.152	2.150
High resolution limit [Å]	2.110	2.110
Rmerge	0.150	2.021
Number of reflections	89266
<I/σ(I)>	9	0.7
Completeness [%]	99.9	99.6
Redundancy	6.5	4.7
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	100 mM Tris-HCl pH 8.5 14% PEG 3350 200 mM A.S.