5LG8
Human L-type ferritin iron loaded for 60 minutes
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9184 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 151.420, 151.420, 151.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.700 - 1.980 |
| R-factor | 0.143 |
| Rwork | 0.142 |
| R-free | 0.17700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fg4 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.214 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.092 | 0.460 |
| Number of reflections | 20846 | |
| <I/σ(I)> | 16.8 | 4.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 11.6 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 281 | 100 mM Cd(NO3)2, 100 mM ammonium sulfate, and 200 mM sodium acetate pH 5 |
